Domain-swapping of mesophilic xylanase with hyper-thermophilic glucanase
نویسندگان
چکیده
منابع مشابه
Domain-swapping of mesophilic xylanase with hyper-thermophilic glucanase
BACKGROUND Domain fusion is limited at enzyme one terminus. The issue was explored by swapping a mesophilic Aspergillus niger GH11 xylanase (Xyn) with a hyper-thermophilic Thermotoga maritima glucanase (Glu) to construct two chimeras, Xyn-Glu and Glu-Xyn, with an intention to create thermostable xylanase containing glucanase activity. RESULTS When expressed in E. coli BL21(DE3), the two chime...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2012
ISSN: 1472-6750
DOI: 10.1186/1472-6750-12-28